Heavy metal bioaccumulation in selected medicinal plants collected from Khetri copper mines and comparison with those collected from fertile soil in Haridwar,India

Heavy metal distribution in medicinal plants is gaining importance not only as an alternative medicine, but also for possible concern due to effects of metal toxicity. The present study has been focused on emphasizing the heavy metal status and bioaccumulation factors of V, Mn, Fe, Co, Cu, Zn, Se (essential metals) and Cr, Ni, Cd, As and Pb (potentially toxic metals) in medicinal plants grown under two different environmental conditions e.g., near to Khetri copper mine and those in fertile soils of Haridwar, both in India, using Instrumental Neutron Activation Analysis (relative method) and Atomic Absorption Spectrometry. The copper levels in the medicinal plants from Khetri were found to be 3-4 folds higher (31.6–76.5 mg kg^?1) than those from Haridwar samples (7.40–15.3 mg kg^?1), which is correlated with very high copper levels (763 mg kg^?1) in Khetri soil. Among various heavy metals, Cr (2.60–5.92 mg kg^?1), Cd (1.47–2.97 mg kg^?1) and Pb (3.97–6.63 mg kg^?1) are also higher in concentration in the medicinal plants from Khetri. The essential metals like Mn (36.4–69.3 mg kg^?1), Fe (192–601 mg kg^?1), Zn (24.9–49.9 mg kg^?1) and Se (0.13–0.91 mg kg^?1) and potentially toxic metals like Ni (3.09–9.01 mg kg^?1) and As (0.41–2.09 mg kg^?1) did not show much variations in concentration in the medicinal plants from both Khetri and Haridwar. The medicinal plants from Khetri, e.g., Ocimum sanctum, Cassia fistula, Withania somnifera and Azadirachta Indica were found rich in Ca and Mg contents while Aloe barbadensis showed moderately high Ca and Mg. Higher levels of Ca-Mg were found to correlate with Zn (except Azadirachta Indica). The bioaccumulation factors (BAFS) of the heavy metals were estimated to understand the soil-to-plant transfer pattern of the heavy metals. Significantly lower BAF values of Cu and Cr were found in the medicinal plants from Khetri, indicating majority fraction of these metals are precipitated and were immobilized species unsuitable for plant uptake. Overall, Withania somnifera (Ashwagandha) showed very high metal bioaccumulation.     

Impact of water quality on the stress physiology of cultured Labeo rohita (Hamilton-Buchanan)

Investigation was conducted for 12 months in two water bodies, S1 with optimum water quality and S2 receiving sewage water. The water quality parameters were assessed in relation to the impact on the stress sensitive physiological parameters of fish Labeo rohita. While optimum levels of transparency, dissolved oxygen, unionised ammonia, alkalinity and hardness in S1 reflected in minimum variation of the physiological parameters of L. rohita but suboptimal levels of DO (nil-18.0 mg/l) and CO2 (nil-16.0 mg/l) observed diurnally and unionised ammonia (0.11-0.42 mg/l) found throughout the experimental period, resulted in significant variation in plasma cortisol (90.0-377.0 ng/ml), cholesterol (89.6-285.0 mg/dl) and condition factor (0.7-1.3) in L. rohita. The results are of significance for fish aquatic habitat management.     

PCB exposure and potential future cancer incidence in Slovak children: an assessment from molecular finger printing by Ingenuity Pathway Analysis (IPA®) derived from experimental and epidemiological investigations

The risk of cancer due to PCB exposure in humans is highly debated. In eastern Slovakia, high exposure of the population to organochlorines (especially PCBs) was associated with various disease and disorder pathways, viz., endocrine disruption, metabolic disorder & diabetes, and cancer, thereby disturbing several cellular processes, including protein synthesis, stress response, and apoptosis. We have evaluated a Slovak cohort (45-month children, at lower and higher levels of PCB exposure from the environment) for disease and disorder development to develop early disease cancer biomarkers that could shed new light on possible mechanisms for the genesis of cancers under such chemical exposures, and identify potential avenues for prevention.

Microarray studies of global gene expression were conducted from the 45-month-old children on the Affymetrix platform followed by Ingenuity Pathway Analysis (IPA®) to associate the affected genes with their mechanistic pathways. High-throughput qRT-PCR TaqMan low-density array (TLDA) was performed to further validate the selected genes on the whole blood cells of the most highly exposed children from the study cohort (n = 71).

TP53, MYC, BCL2, and LRP12 differential gene expressions suggested strong relationships between potential future tumor promotion and PCB exposure in Slovak children. The IPA analysis further detected the most important signaling pathways, including molecular mechanism of cancers, prostate cancer signaling, ovarian cancer signaling, P53 signaling, oncostatin M signaling, and their respective functions (viz., prostate cancer, breast cancer, progression of tumor, growth of tumor, and non-Hodgkin’s disease). The results suggest that PCB exposures, even at the early age of these children, may have lifelong consequences for the future development of chronic diseases.

     

Static and Dynamic Behavior of Fibrous Polymeric Composite Materials at Different Environmental Conditions

The study of static and dynamic behavior of environmentally conditioned fibre reinforced polymeric (FRP) composites is necessary and crucial to examine the durability, reliability and sustainability of these noble materials. FRP composites are being used all around the globe and substituting the conventional materials, starting from mini toys to large aerospace components. Present review has introduced to accumulate and understand the disseminate literature in concentrating the significance of understanding the static and dynamic behavior of FRPs with changing environmental conditionings (hygrothermal, low and high temperature, salt solution, freeze thaw, UV light) and with the interaction of different nano-fillers. Their stability and integrity in diverse service environments may be reformed by their reactions against different nature of loadings i.e. static or dynamic and the components such as fibre, matrix and fibre/matrix interfaces in those environments. The static and dynamic states of loading may come with a possible weaker region to encounter the durability and integrity of the composites. To understand the exact failure modes that correlates the position of environmentally conditioned interfaces and dynamic state of loadings, thus confusing the estimation of its overall performance and mechanical behavior. Interface reliability and durability is vital since in-service environments the degradation in the interfacial region leads to complete composite failure. Therefore, the study of combined effects of various in-service environmental conditions and the role of static and dynamic behavior on the interface will be a crucial part related to the multiaxial dynamic states of failures occurring in FRP’s.     

Use of water quality index and multivariate statistical techniques for the assessment of spatial variations in water quality of a small river

Rapid urban development has led to a critical negative impact on water bodies flowing in and around urban areas. In the present study, 25 physiochemical and biological parameters have been studied on water samples collected from the entire section of a small river originating and ending within an urban area. This study envisaged to assess the water quality status of river body and explore probable sources of pollution in the river. Weighted arithmetic water quality index (WQI) was employed to evaluate the water quality status of the river. Multivariate statistical techniques namely cluster analysis (CA) and principal component analysis (PCA) were applied to differentiate the sources of variation in water quality and to determine the cause of pollution in the river. WQI values indicated high pollution levels in the studied water body, rendering it unsuitable for any practical purpose. Cluster analysis results showed that the river samples can be divided into four groups. Use of PCA identified four important factors describing the types of pollution in the river, namely (1) mineral and nutrient pollution, (2) heavy metal pollution, (3) organic pollution, and (4) fecal contamination. The deteriorating water quality of the river was demonstrated to originate from wide sources of anthropogenic activities, especially municipal sewage discharge from unplanned housing areas, wastewater discharge from small industrial units, livestock activities, and indiscriminate dumping of solid wastes in the river. Thus, the present study effectively demonstrates the use of WQI and multivariate statistical techniques for gaining simpler and meaningful information about the water quality of a lotic water body as well as to identify of the pollution sources.     

Development of ground vegetation under exotic tree plantations on restored coal mine spoil land in a dry tropical region of India

Restoration of mine spoil is a prime need for coal industry. The study of ground cover vegetation provides essential information about the species diversity and their successional trends during the restoration. The present study was conducted to analyze the structure and biomass accumulation of ground vegetation developing in different plantation stands of an opencast coal mine spoil in a dry tropical region. Different plantation stands showed variations in species diversities. Exotic herbs were more dominant in comparison to native herbs. Pennisetum pedicillatum, an exotic herb showed maximum Importance Value Index in most of the plantation stands. Total number of species varied between 12-18 in different plantation stands. Speces richness and evenness increased with increasing age of the plantations. Variations in total biomass accumulation of ground vegetation were also significant among different plantations. These results suggest that reforestation programme with exotic species on coal mine spoil has been successful in colonization of ground vegetation under different plantations. Gravellia pteridifolia plantations showed most successful ground cover among different plantation stands.     

Histological examination of sublethal effects of diazinon on ovary of bluegill,Lepomis macrochirus

The effects of the insecticide, diazinon (an organophosphorous compound), on the ovaries of bluegill (Lepomis macrohirus) were studied. Histological preparations of bluegill ovarian tissue was examined at 24, 48, 72 and 96 h and 1, 2 and 3 weeks following exposure to sublethal doses of diazinon (60 microg/l). The control contained primary follicles with an intact ovigerous lamellae and tunica albuginea. The control also revealed well developed stage IV oocytes with properly distributed provitelline nucleoli. After 24 h of exposure to diazinon, primary follicles began to show adhesion and cytoplasmic retraction in oocyte II occured. Cytoplasmic degeneration and additional adhesion and more retraction were visible at 48 h in oocyte II; 72 h brought forth adhesion, retraction and cytoplamic expulsion from oocyte IV. The number of atretic oocytes increased. Damages to the oocyte IV started to occur after 72 h of exposure. Cytoplasmic retraction and clumping was more visible at 96 h in oocyte IV. Partial destruction of the ovigerous lamellae and vitellogenic membrane occurred after 1 week. Two weeks continued to reveal destruction of follicles. Severe damage of the ovigerous lamellae, increased intrafollicular spaces, vacuolated cytoplasm, extrusion of karyoplasm and necrosis in the cytoplasm were most evident following 3 weeks of exposure. The ovarian wall became frayed and broken. Additionally, a marked increase of atretic follicles, shrinkage, and embedded nucleoli into the surrounding cytoplasm in oocyte II, III and IV were observed at week 3. This study revealed that oocytes at their different stages of maturation get affected differently at various exposure. Based on observations of the ovarian tissue compositional and structural changes following given exposure times, it becomes evident that consistent sublethal doses of diazinon can and will alter microscopic anatomy of the fish ovary.     

Sublethal effects of diazinon on the structure of the testis of bluegill,Lepomis macrochirus: a microscopic analysis

A histopathological analysis of the testis exposed to diazinon, an organophosphorus pesticide was conducted. The testis from the control fish exhibited a normal histological structure with a round, oval or somewhat rectangular shaped with closely associated seminiferous tubules. The seminiferous tubules were surrounded by a layer of connective tissue with myoid cells, Leydig cells, blood vessels, lymph vessels and nerves. Upon exposure for 24 h, the overall structure of the testis looked disrupted and more often the sperm cells appeared to be somewhat larger, but spermatid were smaller than that of the control group. The 48 h exposure caused more dense and closely associated seminiferous tubules. The 72 h exposure induced dilation of the seminiferous tubules and their lumen. Ninety-six hours exposure induced more loose and disrupted structures. In this group (96 h), there was a significant reduction in both lumen and seminiferous tubule size in comparison with the control, 24, 48, and 72 h groups. After 2 weeks of exposure hardly any lumen was seen, which imparted more dense and compacted structures. The biometric analysis gave a highly significant increase in the diameter of the seminiferous lumen. After 48 h of exposure a significant reduction was seen in diameter in comparison with that of the control group. Seventy-two hour exposure indicated an increase in diameter, whereas 96 h exhibited another decrease. One week caused the diameter to increase whereas 2 weeks of exposure caused them to decrease again. The increase in diameter of the seminiferous tabule may be caused by weakening of the surrounding connective tissue and myoid cells, due to exposure to diazinon. The change in the diameters of the seminiferous tabules was very irregular. No apparent co-relation was found between the size of the fish, body weight and weight of the testis after they have been subjected to different exposure periods. There was significant change in the germ cells' diameters. As the germ cells are the essential first step in the process of spermatogenesis, their size reduction may hinder the production of viable spermatozoa, endangering the population dynamics. In order to find out the pathways of this pesticide effects, we propose to study the histopathology of pituitary gland a vital organ of the reproductive axis.     

Shoreline shifting and its prediction using remote sensing and GIS techniques: a case study of Sagar Island,West Bengal (India)

The shoreline position is difficult to predict but the trend of erosion or accretion can be determined by geospatial and statistical techniques which may help in reducing the loss of property. The study aims to assess the shoreline changes and prediction in Sagar Island, a delta of the Ganges, situated in West Bengal, India. Shorelines have been delineated by using Tasseled Cap Transformation techniques from the Landsat MSS (1975), Landsat TM (1989, 1991) and Landsat ETM+ (1999, 2002, 2005, 2008, and 2011) images. The uncertainty was calculated for every year for assessing the positional error related to shoreline extraction. Total shoreline change rate/year has also been calculated and the uncertainty of total shoreline change rate was found ±3.20 m/year. In the present study, End Point Rate (EPR) and Linear Regression (LR) methods have been used for shoreline change rate calculation and prediction of shoreline. Long term (1975–2002) and short term (2002–2011) erosion and accretion rates were calculated for the study area. Sagar is the biggest island of the Sundarban region; so, it was divided into three segments in order to analyze the change on a segment basis. It was observed that the most of erosion occurred in the Segment B (south Sagar). The rate of erosion was ?7.91 and ?7.01 m/year for the periods 1975–2002 and 2002–2011 respectively. The mean shoreline change rate was high in Segment B (South Sagar) with values of ?6.46 m/year (1975–2002) but the rate was decreased into ?5.25 m/year during the later period (2002–2011). The study reveals that most of the southern part of Sagar Island is vulnerable to high rate of shoreline erosion.